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Three representative trees were selected from each tree species. Four fully-grown healthy leaves were picked in different directions of each tree. SPAD-502 chlorophyll meter was used to measure SPAD values. Six points were evenly selected on each leaf for observation. , Calculate the average. The leaves measured by the chlorophyll meter were quickly removed and placed in a portable ice box to test the chlorophyll content in the laboratory. The unit fresh weight and unit area chlorophyll content were calculated, including chlorophyll a, chlorophyll b, and total chlorophyll content. Repeat 3 times for each tree species.
The chlorophyll content range of the fresh green leaves of the main tree species in northwestern Liaoning ranged from 0.73 to 4.61 mg.g-1. The coefficient of variation was 43. 6%, of which the smallest of the pines, the largest in the country; the change in the chlorophyll content per unit area is 0. 21 to 0. 99 g・m-2, the coefficient of variation is 35. 2%, of which the smallest was A. sinica, and the most was Acacia; the SPAD value range was 29. 42 to 75. 51, the coefficient of variation is 23. 0%, the smallest weeping willow, the largest forsythia.
There was a significant positive correlation between paddle leaf values ​​and the chlorophyll a content per unit of fresh green tree species in northwestern Liaoning (R = 0.30, P = 0.04, and there was no significant correlation with the unit fresh weight chlorophyll b content and total chlorophyll content. The value of the padding was significantly positively correlated with the content of chlorophyll a, chlorophyll b and total chlorophyll per unit area.The above correlation analysis was first determined by the chlorophyll meter directly, and then linked to the computer for analysis by software.
Chlorophyll is the basic material for photosynthesis of green plants. It is closely related to the photosynthetic rate. In photosynthesis, it participates in energy transfer in the form of electron transfer and resonance and converts light energy into chemical energy. Chlorophyll content is an important observational parameter in plant physiological and biochemical studies and ecological surveys. It can be used to express plant biomass, detect plant health and response plant adaptation to the environment. In order to accurately obtain chlorophyll content, the instrument method of chlorophyll meter is commonly used. measuring.