The definition of serum: Serum category: The main role of serum: Serum preservation: Activated Alumina,Activated Alumina Balls,Alumina Desiccant,Alumina Adsorbent Ningbo Cijie Chemical Equipment Co., Ltd. , https://www.shengjie-tower.com
Serum refers to blood plasma that has been removed from the blood plasma by the removal of fibrin-derived light yellow transparent liquid or by the removal of fibrin. Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding proteins, provide pro-contact and extension factors to adhere cells to mechanical damage, and provide some protection to cells in culture.
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â—Provide basic nutrients: amino acids, vitamins, inorganic substances, lipids, nucleic acid derivatives, etc., which are necessary for cell growth.
â— Provide hormones and various growth factors: insulin, corticosteroids, steroid hormones (estradiol, testosterone, progesterone) and so on. Growth factors such as fibroblast growth factor, epidermal growth factor, platelet growth factor and the like.
• Providing binding proteins: The role of binding proteins is to carry important low-molecular weight substances such as albumin carrying vitamins, fats, and hormones, and transferrin carrying iron. Bound proteins play an important role in cell metabolism.
• Providing pro-contact and stretch factors to adhere cells to mechanical damage.
â— It has some protective effects on cells in culture: Some cells, such as endothelial cells and bone marrow-like cells, can release proteases, and serum contains anti-protease components, which play a role in neutralization. This effect was discovered by chance, and now there is a purposeful use of serum to stop trypsin digestion. Because trypsin has been widely used for the digestion and passage of adherent cells. Serum proteins form the viscosity of serum, which protects cells from mechanical damage, especially when stirred in suspension culture. Viscosity plays an important role. The serum also contains trace elements and ions, which play an important role in metabolic detoxification, such as SeO3, selenium, etc.
Serum should be kept at -5. C to -2O. C. Store in 4. At C, the shelf life is reduced to one month. If you are unable to use a bottle at a time, it is recommended that the serum be aseptically filled into an appropriate sterile container and then returned to the freezer.